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Test Code DAT Direct Coombs, Blood


Shipping Instructions


Specimen must arrive within 48 hours of collection.



Specimen Required


Container/Tube: Pink top (EDTA)

Specimen Volume: Entire specimen

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Useful For

Detection of alloantibodies or autoantibodies directed against red blood cell antigens

 

Evaluation of transfusion reactions

 

Evaluation of hemolytic anemia

Testing Algorithm

If the direct Coombs result is positive due to IgG, then antibody elution will be performed at an additional charge.

The following tests may also be ordered and performed as part of a positive direct Coombs: monospecific direct Coombs, antibody elution, antibody identification, and antibody absorption.

Method Name

DAT: Direct Antiglobulin Test

ABID: Antibody Identification

ELU: Acid-EDTA Elution

IGGC: Direct Antiglobulin Test (Monospecific)

C3AHG: Hemagglutination

Reporting Name

Direct Coombs

Specimen Type

Whole Blood EDTA

Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient (preferred) 4 days
  Refrigerated  14 days

Reject Due To

Gross hemolysis Reject

Reference Values

Negative

If positive due to IgG, antibody elution will be performed.

Performing Laboratory

MCHS- Mankato

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86880-Direct Coombs

86860-Antibody elution (if appropriate)

86870-Antibody identification (if appropriate)

86978-Absorption (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DAT Direct Coombs 1007-4

 

Result ID Test Result Name Result LOINC Value
DAT Direct Coombs 1007-4

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 day