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HelpTest Code GENS Bacterial Culture, Aerobic with Antimicrobial Susceptibilities, Varies
Shipping Instructions
Specimen must arrive within 24 hours of collection. Ship ambient or refrigerated. Frozen specimens will be rejected.
Necessary Information
Specimen source is required; include the specific anatomic source. Indicate whether it is a "surface" or "deep/surgical" specimen. Do not label only as "wound."
Specimen Required
Preferred:
Specimen Type: Closed abscess; deep tissue or fluid
Container/Tube: Sterile container
Specimen Volume: Entire collection
Collection Instructions: Aspirate the abscess contents/fluid with a syringe or excise a portion of tissue.
Acceptable:
Specimen Type: Open abscess, superficial lesion/wound, swab, tissue, or fluid
Supplies:
-Culturette (BBL Culture Swab) (T092)
-BD E-Swab (T853)
Container/Tube: Sterile container, culture transport swab (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium) or ESwab
Collection Instructions: For most open lesions and abscesses, remove superficial microbiota by decontaminating skin before collecting a specimen from advancing margin or base.
Additional Information:
1. If submitting a specimen from a source contaminated with usual microbiota, send at refrigerated temperature.
2. Refrigerated specimens are not suitable for isolation of Neisseria species.
Useful For
Detecting bacteria responsible for infections of sterile body fluids, tissues, or wounds
Determining the in vitro antimicrobial susceptibility of potentially disease-causing aerobic bacteria, if appropriate
This test is not intended for medicolegal use.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| GID | Bacteria Identification | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| SALS | Serologic Agglut Method 1 Ident | No, (Bill Only) | No |
| EC | Serologic Agglut Method 2 Ident | No, (Bill Only) | No |
| SIDC | Ident Serologic Agglut Method 4 | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| PCRID | Identification by PCR | No, (Bill Only) | No |
| BLA | Beta Lactamase | No, (Bill Only) | No |
| MIC | Susceptibility, MIC | No, (Bill Only) | No |
| SUS | Susceptibility | No, (Bill Only) | No |
| HPCR1 | H pylori + Clarithro Resistance PCR | No, (Bill Only) | No |
| MECAB | mecA PCR Test, Bill Only | No, (Bill Only) | No |
| CARNB | Carbapenemase-Carba NP Test | No, (Bill Only) | No |
| CRPCB | CARBA- R PCR, Bill Only | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge. Antimicrobial agents appropriate to the organism and specimen source will be tested according to Mayo Clinic's practice and the laboratory's standard operating procedures.
The following tables provide a listing of the antimicrobials routinely tested in the laboratory as well as antimicrobials that may be tested upon request. These tables are organized by isolate groups and are not all inclusive. Call 800-533-1710 and ask to speak to the Bacteriology Antimicrobial Susceptibility Testing Laboratory if the organism or antimicrobial of interest are not listed in these tables.
-Aerobic Gram-Negative Bacilli Antimicrobials
-Additional Gram-Negative Bacteria Antimicrobials
-Staphylococcus, Enterococcus, Bacillus, and Related Genera Antimicrobials
Special Instructions
Method Name
Conventional Culture Technique with Minimal Inhibitory Concentration (MIC) (Agar Dilution or Broth Microdilution or Gradient Diffusion) or Disk Diffusion (if appropriate)
Reporting Name
Bacterial Culture, Aerobic + SuscSpecimen Type
VariesSpecimen Minimum Volume
0.5 mL or 0.5 × 0.2 × 0.2-cm sized piece of tissue
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | |||
Reject Due To
| Dry swab | Reject |
Reference Values
No growth or usual microbiota, Identification of probable pathogens
Susceptibility results are reported as minimal inhibitory concentration (MIC) in mcg/mL. Breakpoints (also known as clinical breakpoints) are used to categorize an organism as susceptible, susceptible-dose dependent, intermediate, resistant, or nonsusceptible according to breakpoint setting organizations, either the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST), as applicable.
In some instances, an interpretive category cannot be provided based on available data; therefore, the following comment will be included on the report: There are no established interpretive guidelines for agents reported without interpretations.
For information regarding CLSI and EUCAST susceptibility interpretations, see Susceptibility Interpretative Category Definitions.
Performing Laboratory
MCHS- MankatoTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87070-Bacterial Culture, Aerobic
87077-Identification commercial kit (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
87077-Bacteria Identification (if appropriate)
87077-Additional Identification procedure (if appropriate)
87077-Identification Staphylococcus (if appropriate)
87077-Identification Streptococcus (if appropriate)
87147 x 1-3-Serologic agglut method 1 ident (if appropriate)
87147-Serologic agglut method 2 ident (if appropriate)
87147 x 4-Serologic agglut method 3 ident (if appropriate)
87147 x 2-6-Serologic Agglut Method 4 Ident (if appropriate)
87153-Aerobe ident by sequencing (if appropriate)
87150-Identification by PCR (if appropriate)
87176-Tissue Processing (if appropriate)
87185-Beta lactamase (if appropriate)
87186-Antimicrobial Susceptibility, Aerobic Bacteria, MIC-per organism for routine battery (if appropriate)
87181-Susceptibility per drug and per organism for drugs not in routine battery (if appropriate)
87150-H pylori + Clarithro Resistance PCR (if appropriate)
87150-mec A PCR (if appropriate)
87185-Carbapenemase detection, Carba NP test (if appropriate)
87150 x 5 Carbapenem resistance genes (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| GENS | Bacterial Culture, Aerobic + Susc | 634-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| GENS | Bacterial Culture, Aerobic + Susc | 634-6 |
Day(s) Performed
Monday through Sunday