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Test Code HV2QU HIV-2 RNA Detection and Quantification, Real-Time qRT-PCR, Plasma


Ordering Guidance


This test is indicated for either 1) confirmatory qualitative detection of HIV-2 RNA in individuals with indeterminate or positive HIV-2-specific antibody but negative HIV-1-specific antibody test results; or 2) baseline or serial monitoring of HIV-2 viral load in those with confirmed HIV-2 infection with or without antiretroviral therapy.



Shipping Instructions


Ship specimen frozen on dry ice. If shipment will be delayed for more than 24 hours, freeze specimen at -20 to -80° C (up to 35 days) before shipment and then transport on dry ice.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 2.1 mL Plasma

Collection Instructions: Centrifuge and aliquot plasma into a plastic vial.


Secondary ID

623478

Useful For

Confirmatory detection of human immunodeficiency virus type 2 (HIV-2) RNA in clinical plasma specimens for the diagnosis of acute or established HIV-2 infection

 

Quantifying plasma HIV-2 RNA levels (viral load) in individuals living with HIV-2:

-Before initiating antiretroviral therapy to obtain baseline viral load

-For monitoring HIV-2 disease progression with or without antiretroviral therapy

Method Name

Reverse Transcription Real-time Polymerase Chain Reaction (RT-qPCR)

Reporting Name

HIV-2 RNA Detect / Quant, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

Plasma: 1.1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen (preferred) 60 days ALIQUOT TUBE
  Refrigerated  6 days ALIQUOT TUBE

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

Undetected

Day(s) Performed

Varies (once per week)

Report Available

1 to 14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87539

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HV2QU HIV-2 RNA Detect / Quant, P 69354-9

 

Result ID Test Result Name Result LOINC Value
623478 HIV-2 RNA Detect / Quant, P 69354-9