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Test Code LH Luteinizing Hormone (LH), Serum

Reporting Name

Luteinizing Hormone (LH), S

Useful For

An adjunct in the evaluation of menstrual irregularities


Evaluating patients with suspected hypogonadism


Predicting ovulation


Evaluating infertility


Diagnosing pituitary disorders

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Specimen Required

Patient Preparation: Twelve hours before this blood test, do not take multivitamins or dietary supplements containing biotin (vitamin B7) that are commonly found in hair, skin and nail supplements and multivitamins.


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.6 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values


0-14 days: not established

15 days-10 years: 0.3-2.8 IU/L

11 years: 0.3-1.8 IU/L

12 years: 0.3-4.0 IU/L

13 years: 0.3-6.0 IU/L

14 years: 0.5-7.9 IU/L

15-16 years: 0.5-10.8 IU/L

17 years: 0.9-5.9 IU/L

≥18 years: 1.8-8.6 IU/L



Stage l: 0.3-2.7 IU/L

Stage ll: 0.3-5.1 IU/L

Stage lll: 0.3-6.9 IU/L

Stage lV: 0.5-5.3 IU/L

Stage V: 0.8-11.8 IU/L

*Puberty onset occurs for boys at a median age of 11.5 (± 2) years. For boys there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.



0-14 days: not established

15 days-3 years: 0.3-2.5 IU/L

4-6 years: ≤1.9 IU/L

7-8 years: ≤3.0 IU/L

9-10 years: ≤4.0 IU/L

11 years: ≤6.5 IU/L

12 years: 0.4-9.9 IU/L

13 years: 0.3-5.4 IU/L

14 years: 0.5-31.2 IU/L

15 years: 0.5-20.7 IU/L

16 years: 0.4-29.4 IU/L

17 years: 1.6-12.4 IU/L

≥18 years


Follicular: 2.1-10.9 IU/L

Midcycle: 20.0-100.0 IU/L

Luteal: 1.2-12.9 IU/L

Postmenopausal: 10.0-60.0 IU/L



Stage I: ≤2.0 IU/L

Stage II: ≤6.5 IU/L

Stage III: 0.3-17.2 IU/L

Stage IV: 0.5-26.3 IU/L

Stage V: 0.6-13.7 IU/L

*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (± 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.


Pediatric ranges derived for DXI method from analytic comparison to reference method in: Elmlinger MW, Kuhnel W, Ranke MB: Reference ranges for serum concentrations of lutropin (LH), follitropin (FSH), estradiol (E2), prolactin, progesterone, sex hormone-binding globulin (SHBG), dehydroepiandrosterone sulfate (DHEAS), cortisol and ferritin in neonates, children and young adults. Clin Chem Lab Med 2002;40(11):1151-1160


For SI unit Reference Values, see

Day(s) and Time(s) Performed

Monday through Friday; 5 a.m.–12 a.m., Saturday; 6 a.m.–6 p.m.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
LH Luteinizing Hormone (LH), S 10501-5


Result ID Test Result Name Result LOINC Value
LH Luteinizing Hormone (LH), S 10501-5

Reject Due To


Mild OK; Gross reject


Mild OK; Gross OK





Method Name

Immunoenzymatic Assay 


If not ordering electronically, complete, print, and send an Oncology Test Request Form (T729) with the specimen (