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Test Code PLHBB Plasma Free Hemoglobin, Plasma

Reporting Name

Plasma Free Hemoglobin, P

Useful For

Determining whether hemolysis is occurring such as from:

-Transfusion reaction

-Mechanical fragmentation of red blood cells

-Relative comparison to baseline levels in extracorporeal membrane oxygenation and centrifugal ventricular assist device patients to assess pump disruption

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Plasma EDTA


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL Plasma

Collection Instructions:

1. Within 2 hours of collection, centrifuge and aliquot plasma into a plastic vial.

2. Results could be falsely elevated due to artifactual red blood cell lysis if not centrifuged within 2 hours of collection.


Specimen Minimum Volume

Plasma: 1.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Refrigerated (preferred) 20 days
  Frozen  30 days
  Ambient  4 days

Reference Values

Total plasma hemoglobin

≥12 months: 0.0-15.2 mg/dL

Reference values have not been established for patients younger than 12 months.

 

Oxyhemoglobin

≥12 months: 0.0-12.4 mg/dL

Reference values have not been established for patients younger than 12 months.

Day(s) Performed

Monday through Sunday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83051

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLHBB Plasma Free Hemoglobin, P 87433-9

 

Result ID Test Result Name Result LOINC Value
31970 Total Plasma Hemoglobin 721-1
31971 Oxyhemoglobin 87437-0

Report Available

1 to 3 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Method Name

Spectrophotometry (SP)

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.

Secondary ID

9096