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Test Code TVRNA Trichomonas vaginalis, Nucleic Acid Amplification, Varies

Useful For

Detecting Trichomonas vaginalis in urine, cervical/endocervical or vaginal specimen types

Reporting Name

Trichomonas vaginalis Amplified RNA

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Endocervix/Cervix

Supplies: Aptima Unisex Swab Collection Kit (T583)

Container/Tube: Aptima Unisex Swab

Specimen Volume: Swab

Collection Instructions:

1. Specimens must be collected using the Aptima Unisex Swab Collection Kit.

2. Use cleaning swab (white shaft) to remove excess mucus from endocervix/cervix.

Discard the cleaning swab.

3. Insert second swab (blue shaft) 1 to 1.5 cm into endocervical canal, and rotate swab gently for 30 seconds. Avoid touching vaginal wall when removing swab.

4. Place second swab (blue shaft) into Aptima transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.

5. Cap tube securely and label tube with patient's entire name and collection date and time.

6. Maintain swab container at 2 to 30° C (refrigerate temperature is preferred), transport within 60 days of collection.

 

Specimen Type: Vaginal

Supplies: Aptima Multitest Swab Collection Kit (T584)

Container/Tube: Aptima Multitest Swab

Specimen Volume: Swab

Collection Instructions:

1. Specimens must be collected using the Aptima Multitest Swab Collection Kit.

2. Insert swab (pink shaft) about 5 cm past introitus and rotate gently for 30 seconds.

3. Place swab into Aptima transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.

4. Cap tube securely and label tube with patient's entire name and collection date and time.

5. Maintain swab container at 2 to 30° C (refrigerate temperature is preferred), transport within 60 days of collection.

 

Specimen Type: ThinPrep Specimen (Endocervix/Cervix)

Supplies: Aptima Thin Prep Transport Tube (T652)

Container/Tube: Thin Prep Transport Tube

Specimen Volume: 1 mL

Collection Instructions:

1. Collect ThinPrep sample as per normal collection process.

2. ThinPrep specimen must be aliquoted (as outlined below) before it is processed/tested for Pap smear.

3. Vortex ThinPrep/PreservCyt vial 3 to 10 seconds. Within 1 minute of vortexing:

a. Transfer 1 mL of specimen into the Aptima specimen transfer tube using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug).

b. Process only 1 ThinPrep and transfer tube set at a time.

c. Recap Aptima specimen transfer tube tightly and gently invert 3 times to mix.

3. Label Aptima transfer tube with appropriate label.

4. Use remainder of ThinPrep specimen for Pap testing.

5. Maintain specimen transport tube at 2 to 30° C (refrigerate temperature is preferred), transport within 30 days of collection when stored at 2 to 14° C or within 14 days when stored at 15 to 30° C.

 

Specimen Type: Urine

Supplies: Aptima Urine Transport Tube (T582)

Container/Tube: Aptima urine specimen transport tube

Specimen Volume: 15 to 20 mL

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to specimen collection.

2. Patient should collect first portion of random voided urine (first part of stream) into a sterile, plastic, preservative-free container.

3. Within 24 hours of collection, transfer 2 mL of urine into the Aptima urine specimen transport tube using the disposable pipette provided. The correct volume of urine has been added when the fluid level is between the black fill lines on the Aptima urine transport tube.


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) APTIMA VIAL
  Frozen  180 days APTIMA VIAL
  Ambient  APTIMA VIAL

Reject Due To

Midstream urine specimen
Clean catch urine specimen
Over-filled or under-filled urine transport tubes
Specimen collected into a SurePath device
Transport tubes containing a cleaning swab or more than 1 swab
Reject

Reference Values

Negative

Day(s) Performed

Monday through Sunday

Report Available

1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87661

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TVRNA Trichomonas vaginalis Amplified RNA 46154-1

 

Result ID Test Result Name Result LOINC Value
SRC29 SOURCE: 31208-2
34810 Trichomonas vaginalis amplified RNA 46154-1

Method Name

Transcription-Mediated Amplification

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Secondary ID

61755