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Test Code XMAHG Crossmatch, Antihuman Globulin (AHG)

Useful For

Detection of allo- or autoantibodies directed against donor red blood cell antigens to determine compatibility in the setting of pretransfusion testing

Testing Algorithm

A TS / Type and Screen is required to obtain the specimen necessary for crossmatching. The specimen is good for 3 days, with the day of collection being day zero. The routine testing performed on the type and screen specimen includes: ABO and Rh typing, and antibody screening for unexpected allo antibodies. A positive antibody screen necessitates antibody identification and a determination of the clinical significance of the antibody. A negative antibody screen, with no history of unexpected antibodies, allows the immediate spin crossmatch or electronic crossmatch to be performed if 2 blood types are on record. A positive antibody screen disqualifies the patient for the immediate spin crossmatch and requires the more sensitive antiglobulin phase of testing. The antihuman globulin (AHG) crossmatch is an antiglobulin phase crossmatch that utilizes polyethylene glycol (PEG) as a potentiator.

 

If donor unit incompatibility is detected, then antibody identification may be performed.

 

The following tests may also be ordered and performed as part of antibody identification if donor incompatibility is detected: patient/donor antigen typing, direct Coombs IgG, elution, and adsorptions.

Method Name

Antiglobulin Phase Crossmatch

Reporting Name

Crossmatch

Specimen Type

Whole Blood EDTA

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA  

Reject Due To

Gross hemolysis Reject

Reference Values

Compatible/Negative

Incompatible/positive, antibody identification will be performed.

Performing Laboratory

MCHS- Mankato

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86920-Crossmatch (IS)

86922-Crossmatch (AHG)

86870-Antibody identification (if appropriate)

86880-Antiglobulin, Direct (if appropriate)

86905-Each red cell antigen typing (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
XMAHG Crossmatch 38167-3

 

Result ID Test Result Name Result LOINC Value
XMAHG Crossmatch 38167-3

Day(s) Performed

Monday through Sunday

Report Available

1 day